Qualitative Methods and the Evaluation of Community Renewal Programs in Australia: Towards a National Framework

The emergence of public housing estate renewal programs in Australia in the last decade has been one of the most prominent developments in social housing policy. These programs have undertaken a broad mix of renewal activity, ranging from outright physical redevelopment and stock replacement for sale, to community development type initiatives to improve social and employment outcomes for residents. However, while a number of evaluations of these programs have been undertaken, the development of evaluation methodology has lagged behind that of other countries. This in part is due to the lack of federal government interest or involvement in these programs which are essentially state specific. The article reviews the evaluations that have been undertaken in the last 10 years in Australia and assesses the relative importance of qualitative methodologies in these evaluations. Despite a strong focus among policy makers on value-for-money aspects of renewal, the authors show that qualitative methods have been commonplace, if limited in range, and argue this is a result of both the difficulty of obtaining comparative quantitative information especially when comparing dissimilar programs between states. In this context, qualitative methods are more easily managed by researchers and offer more insightful assessments than quantitatively based approaches. The article concludes by arguing for a national evaluation methodology to assist in more rigorous evaluations and the extension of qualitative evaluation methods.     

Stop that E-mail! You are probably breaking the law!

The author has become very concerned that many sites are not properly adhering to US export regulations in the area of technical data and restricted technical science as prescribed by US law. The author strongly recommends that legal counsel be contacted with the above information and components and documents be put into place at customers to ensure that violation of any export law is not possible and that proper maintenance of restricted entities is maintained.     

Metabolic mapping of the effects of oral alcohol self-administration in rats

A method for the quantitation of protein carbonyls, which have been widely employed as markers of protein oxidative damage, is described. Protein carbonyls were derivatized with tritiated sodium borohydride and the tritiated proteins were separated on SDS-PAGE. Protein bands, visualized by Coomassie blue staining, were then excised and incubated in 30% H2O2 at 60 degrees C for 48 h. Tritium, incorporated into the proteins, was quantitated by liquid scintillation counting after gel solubilization by H2O2. This method can be applied to the measurement of carbonylation of specific proteins as it employs SDS-PAGE and has the advantage that unreacted NaB3H4 in the labeling reaction mixture need not be removed. The present method, when combined with immunochemical detection of protein carbonyls, should be very useful in the quantitation of oxidative damage to individual proteins.     

Toward continued independent living for older Americans.

The author describes his US Senate bill (S. 1244), the Senior Citizens Independent Community Care Act, which would expand the scope of benefits in the Medicare program to include individual assessment and treatment plans, homemaker/home-health aid, adult daycare services, interim care, and any other services that the US Health and Human Resources Department deems necessary. The goal of the bill is to provide home health care to allow individuals to stay with their families or in their communities. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Suggestions for altered auditory sensitivity, the negative subject effect and hypnotic susceptibility: A signal detection analysis.

In Experiment I, using 96 undergraduates, low susceptibles (Harvard Group Scale of Hypnotic Susceptibility, Form A) showed higher sensitivity than high susceptibles, and hypnotic induction failed to affect the sensitivity of either highs or lows. Among the high susceptibles, suggestion to either increase or decrease acuity produced increments in sensitivity and reduced individual differences. Suggestions (regardless of the direction of change suggested) probably motivated high susceptibles, who would have otherwise performed inefficiently, to perform as efficiently as low susceptibles. With respect to response bias, high-susceptible Ss given a suggestion for increased acuity behaved in terms of suggested demands; they showed an increased tendency to respond "signal." Low-susceptible Ss given the same suggestion responded in a counterdemand or "negative subject" fashion; they developed a bias to say "noise." Exp II used a more difficult discrimination task with 80 Ss to replicate and extend this counterdemand effect in low susceptibles. (43 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Direct substitution of polyolefins

Reaction of solutions of ethylene-propylene copolymer with carbonium ion sources, such as isopropylbenzene, in the presence of acid catalysts leads to the introduction of the aromatic and hydroxylic moieties onto the copolymer backbone. Major gaseous products are isobutane and propane. Chain scission accompanies the reaction.     

Injuries to the phrenic nerve resulting in diaphragmatic paralysis with special reference to stretch trauma

The effectiveness of purines and purine analogues as inducers of erythroid differentiation in cultured murine erythroleukemia cells has been investigated. These cell lines have previously been shown to differentiate in vitro in response to dimethylsulfoxide (DMSO) and a number of other polar solvents. Two purine analogues, 6-thioguanine and 6-mercaptopurine, as well as the naturally occuring purine, purine, hypoxanthine, are shown to be extremely potent inducers. 6-Thioguanine is effective at a concentration of 0.06 mM, 750 fold lower than the DMSO concentration required for equivalent induction. 6-Mercaptopurine and hypoxanthine are effective inducers at a concentration of approximately 2 mM. Accumulation of globin mRNA was monitored during induction with purine inducers and shown to be similar in amount to globin mRNA levels reached in DMSO-induced cultures. Induction of differentiation by all three compounds follows a similar time course to induction with DMSO. All three compounds are potent inducers of HGPRT (hypoxanthine-guanine phosphoribosyltransferase)-negative cell lines; hence incorporation of purines into DNA is not required for induction of differentiation. Comparison of these compounds with other purines and purine analogues suggests a high degree of specificity in their interaction with a cellular target.     

A non-lensed fiber-optic resonance-enhanced multiphoton ionization probe

We have developed a miniature fiber-optic probe with no focusing optics for in situ analysis of volatile organic compounds (VOCs). The probe uses an optical fiber to transmit a laser pulse to a vapor sample causing it to ionize adjacent to the fiber tip through a resonance-enhanced multiphoton ionization (REMPI) process. The distal end of the optical fiber is contained co-axially within 2-mm-inner-diameter stainless steel tubing that serves as an electrode. The electrode is biased at a high positive potential to collect electrons. The current generated is shown to be proportional over about two orders of magnitude to the concentration of the species ionized. Visible wavelength REMPI spectroscopy is used to determine probe sensitivities of 20 ppb (benzene) and 43 ppb (toluene). Designing the probe without focusing optics specifies an achromatic ionization region constant in size and position as the laser wavelength is scanned, which simplifies data collection and reduction. Focusing achromatic systems are discussed and the potential signal improvement is estimated.     

Cholinergic vasoconstrictor effects in the rabbit eye: vasomotor effects of pentobarbital anesthesia

Oculomotor nerve stimulation causes vasoconstriction in the anterior uvea, which is due partly to a muscarinic mechanism and partly to a non-sympathetic aminergic mechanism. The labelled microsphere method was used to analyze the effect of pentobarbital anesthesia on the resting cholinergic vasomotor tone in the anterior uvea and to determine the relationship between stimulation frequency and vasomotor response. An attempt was made also to ascertain whether the aminergic part of the vasoconstriction is caused by release of 5-hydroxytryptamine or norepinephrine. Induction of pentobarbital anesthesia caused a marked vasodilation in the iris and the ciliary processes and a subsequent muscarinic blockade had no effect on the blood flow. A similar result was obtained in the optic nerve. In the choroid plexus, heart muscle, pineal body and coecum, pentobarbital anesthesia caused vasodilation and a subsequent muscarinic blockade caused vasoconstriction. In the brain pentobarbital anesthesia caused a marked reduction in the blood flow of the grey matter and a moderate reduction in the white matter. After a muscarinic blockade there was some increase in the blood flow of the grey matter. Stimulation of the oculomotor nerve caused near maximum vasomotor responses at 10-20 Hz; maximum effect on the pupil size was obtained at 40-50 Hz. Depletion of 5-hydroxytryptamine with fenfluramine did not prevent the aminergic part of the vasoconstriction and a marked vasoconstriction was also observed after pretreatment with reserpine. The results indicate the pentobarbital anesthesia abolishes most of the spontaneous cholinergic vasoconstrictor tone of the anterior uvea and that the aminergic part of the oculomotor nerve induced vasoconstriction is caused by the stimulation of phentolamine-sensitive receptors by a mechanism probably not involving release of norepinephrine or 5-hydroxytryptamine.     

Genomic mining for complex disease traits with “random chemistry”

Our rapidly growing knowledge regarding genetic variation in the human genome offers great potential for understanding the genetic etiology of disease. This, in turn, could revolutionize detection, treatment, and in some cases prevention of disease. While genes for most of the rare monogenic diseases have already been discovered, most common diseases are complex traits, resulting from multiple gene–gene and gene-environment interactions. Detecting epistatic genetic interactions that predispose for disease is an important, but computationally daunting, task currently facing bioinformaticists. Here, we propose a new evolutionary approach that attempts to hill-climb from large sets of candidate epistatic genetic features to smaller sets, inspired by Kauffman’s “random chemistry” approach to detecting small auto-catalytic sets of molecules from within large sets. Although the algorithm is conceptually straightforward, its success hinges upon the creation of a fitness function able to discriminate large sets that contain subsets of interacting genetic features from those that don’t. Here, we employ an approximate and noisy fitness function based on the ReliefF data mining algorithm. We establish proof-of-concept using synthetic data sets, where individual features have no marginal effects. We show that the resulting algorithm can successfully detect epistatic pairs from up to 1,000 candidate single nucleotide polymorphisms in time that is linear in the size of the initial set, although success rate degrades as heritability declines. Research continues into seeking a more accurate fitness approximator for large sets and other algorithmic improvements that will enable us to extend the approach to larger data sets and to lower heritabilities.